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镜鲤与建鲤生长性状共享 QTL 标记及优势基因型 [中文引用][英文引用]

Analysis of shared QTLs and preferred genotypes between mirror carp and Jian carp

作者(英文):LU Cuiyun1 3  GU Ying1  LI Chao1  ZHENG Xianhu1  CHENG Lei1  CAO Dingchen1  YU Juhua2  HE Lichuan1  SUN Xiaowen1  
关键词:镜鲤  建鲤  共享 QTL  优势基因型 
关键词(英文):Cyprinus carpio L.  Cyprinus carpio var. jian  shared QTL  preferred genotype 
分类号:S917
出版年·卷·期(页码):2015·22·第3期(0-0)
DOI: 10.3724/SP.J.1118.2015.14293
-----摘要:-------------------------------------------------------------------------------------------

本研究以 1 个镜鲤(Cyprinus carpio L.)全同胞家系(190 个个体)构建的微卫星遗传图谱(992 个标记)为基础, 从体重、体长、体高和体厚的 QTL 区间内发掘了 54 个标记, 其与性状具有显著相关性, 进而通过对不同基因型性 状间的比较, 筛选出 83 个优势基因型。在此基础上, 用 54 个镜鲤 QTL 标记分析了建鲤(Cyprinus carpio var. jian) 作图群体, 其中40个标记在建鲤中表现出多态性, 比例为74.07%; 相关性分析结果显示, 其中22个标记与建鲤家 系的体重、体长、体高或体厚性状具有显著相关性(P<0.05), 占多态标记的 55.00%; 镜鲤与建鲤共享的 22 个 QTL 标记中, 18 个标记与至少 1 个相同的性状具有显著相关性(P<0.05), 从中筛选出建鲤性状具有优势的基因型 30 个, 可用于指导建鲤的选育。品种间共享 QTL 的发掘能够扩展 QTL 标记的使用空间, 减少新品种重新构建图谱进行 QTL 标记定位的工作量和成本。

-----英文摘要:---------------------------------------------------------------------------------------

Common carp (Cyprinus carpio L.) is an important economic fish species with very rich resources, which have been used to develop many carp varieties in China. Because of the significant differences in the genetic background among varieties, quantitative trait loci (QTLs) detected from one variety are difficult to use directly in another variety. In addition, it is impossible to construct a genetic map and detect QTL markers affecting important traits in every variety. Thus, it is a good selection strategy to verify QTLs in one variety and identify shared QTL markers. The methods will save time and money and can be used to detect QTLs quickly in different varieties. Mirror carp (Cyprinus carpio L.) and Jian carp (Cyprinus carpio var. jian) are two important Chinese aquaculture varieties that play an important role in selective breeding. Significant differences in body shape, growth rate, and growth scale have been found between Jian carp and mirror carp. Thousands of QTLs affecting important traits have been detected recently in different mirror carp panels. However, the number of Jian carp QTLs is very limited. In this study, 89 microsatellite markers were selected to identify markers associated with mirror carp traits. These markers were located at overlaps or flanks of QTL interval peaks affecting body weight, standard length, body height, and body thickness based on a genetic map containing 992 microsatellite markers. As a result, 54 QTL markers and 83 preferred genotypes were identified by assessing the contribution of different genotypes on traits. In addition, a mapping population containing 94 Jian carp was analyzed using these 54 QTL markers. Forty markers were polymorphic in Jian carp, with a percentage of 74.07%, which was higher than that of randomly selected markers. Correlation analysis results indicated that 22 markers were shared QTLs between mirror carp and Jian carp, and 18 of the shared markers were significantly correlated with the same trait in the two carp. Thirty preferred genotypes were detected in Jian carp, which will be useful for a marker-associated selection breeding program. The QTLs shared between strains can be used to reduce the effort and cost of QTL mapping in new common carp varieties.

-----参考文献:---------------------------------------------------------------------------------------

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中文著录格式: 鲁翠云1,3,,顾颖1,,李超1,,郑先虎1,,程磊1,,曹顶臣1,,俞菊华2,,何立川1,,孙效文1,.镜鲤与建鲤生长性状共享 QTL 标记及优势基因型.中国水产科学.2015;22(3):0-0.
英文著录格式: LU,Cuiyun1,3,,GU,Ying1,,LI,Chao1,,ZHENG,Xianhu1,,CHENG,Lei1,,CAO,Dingchen1,,YU,Juhua2,,HE,Lichuan1,,SUN,Xiaowen1,.Analysis of shared QTLs and preferred genotypes between mirror carp and Jian carp.No Title Settings.2015;22(3):0-0.

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