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源于鳞被相关基因的微卫星标记与鲤 4 种生长性状的相关性分析 [中文引用][英文引用]

Correlation analysis of microsatellite markers derived from scale genes (ant, eda, edar, fgfr) with four growth traits in common carp (Cyprinus carpio) 

作者: class="p0" style="margin-bottom:0pt  margin-top:0pt  "> style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'方正书宋简体'  ">肖同乾 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'Times New Roman'  vertical-align:super  ">1   2 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'方正书宋简体'  ">    face="方正书宋简体">鲁翠云 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'Times New Roman'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'方正书宋简体'  ">    face="方正书宋简体">李超 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'Times New Roman'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'方正书宋简体'  ">    face="方正书宋简体">曹顶臣 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'Times New Roman'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'方正书宋简体'  ">    face="方正书宋简体">程磊 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'Times New Roman'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'方正书宋简体'  ">    face="方正书宋简体">孙效文 style="mso-spacerun:'yes'  letter-spacing:0.2000pt  font-size:10.5000pt  font-family:'Times New Roman'  vertical-align:super  ">2

 
作者(英文): class="p16" style="margin-bottom:0pt  margin-top:0pt  "> style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'Times New Roman'  ">Xiao style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'方正书宋简体'  ">   style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">Tongqian style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  vertical-align:super  ">1 2 style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">    style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'Times New Roman'  ">Lu style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'方正书宋简体'  ">   style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">Cuiyun style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'方正书宋简体'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">    style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'Times New Roman'  ">Li style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">  Chao   style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'方正书宋简体'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">  style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'Times New Roman'  ">  Cao style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'方正书宋简体'  ">   style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">Dingchen style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'方正书宋简体'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'方正书宋简体'  ">    style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'Times New Roman'  ">Cheng style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">  Lei   style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'方正书宋简体'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">    style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'Times New Roman'  ">Sun style="mso-spacerun:'yes'  letter-spacing:0.0000pt  text-transform:uppercase  font-size:10.5000pt  font-family:'方正书宋简体'  ">   style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  ">Xiaowen style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'方正书宋简体'  vertical-align:super  ">2 style="mso-spacerun:'yes'  letter-spacing:0.0000pt  font-size:10.5000pt  font-family:'Times New Roman'  vertical-align:super  ">

 
关键词(英文):Cyprinus carpio  scale  growth trait  microsatellite marker  correlation analysis 
分类号:S917
出版年·卷·期(页码):2014·21·第5期(883-892)
DOI: 10.3724/SP.J.1118.2014.00883
-----摘要:-------------------------------------------------------------------------------------------

 选用来源于鲤基因组中的鳞被相关基因(ant、eda、edar、fgfr)及其上下游序列中的 155 个微卫星标记, 对德 国镜鲤(Cyprinus carpio L.)与黑龙江野鲤(Cyprinus carpio haematopterus)杂交的 F2 116 尾个体的遗传多样性进行检 测, 以卡方检验估计群体 Hardy-Weinberg 平衡。结果表明, 36 个微卫星标记表现为多态, 各标记的等位基因数在 2~4 个浮动, 共检测到86 个等位基因, 平均每个标记 2.388 9 个; 平均有效等位基因数为 2.209 4; 平均观测杂合度 为 0.624 5; 平均期望杂合度为 0.529 2; 平均多态信息含量为 0.432 1, 其中 23 个微卫星标记表现为中度多态 (0.25≤PIC<0.5), 13 个微卫星标记表现为高度多态(PIC≥0.5), 说明这个群体属于中度多态性水平。Hardy-Weinberg 平衡检验结果显示, 61%标记显著偏离平衡, 人工选择压力和自交对家系造成了严重的影响。SPSS 17.0 分析发现分 别有 10(28%)、7(19%)、7(19%)和 11 个(31%)标记与体质量、体长、体高和体厚存在显著相关性, 并发现 11 个优 势基因型。源于鳞被相关基因的微卫星标记与生长性状连锁的比例较高, 以上结果从分子水平上提示鳞被基因与 所研究 4 种生长性状存在一定的相关性。 

-----英文摘要:---------------------------------------------------------------------------------------

 We evaluated the correlation of scale genes (ant, eda, edar, fgfr) with four growth traits in common carp(Cyprinus carpio). A total of 155 microsatellite markers were selected from the homologous sequence of the carp genome. We used 116 progeny from a self-bred line of F1 derived from intercross lines of German mirror carp (Cyprinus carpio L.) and Amur wild carp (Cyprinus carpio haematopterus). We estimated the following genetic diversity parameters: allele number(No), effective allele number(Ne), observed heterozygosity(Ho), expected heterozygosity(He) using PopGene (Version 1.32). We used achi-square test to test for Hardy–Weinberg equilibrium. Of the 155 markers, 36 microsatellite loci were polymorphic with 2–4 alleles for each locus (86 alleles for the 36 loci). The mean number of alleles and the mean effective number of alleles were 2.388 9 and 2.209 4, respectively. The mean observed heterozygosity and expected heterozygosity were 0.624 5 and 0.529 2, respectively. The mean polymorphism information content (PIC) was 0.432 1 for the 36 loci, of which 23 loci had a moderate level of polymorphism (0.25≤ PIC<0.5) and 13 loci had a high level of polymorphism (PIC≥0.5). Our results suggest the population has a moderate level of polymorphism. Given that the microsatellite markers were located in the genes or their contexts, the level of polymorphism may be even lower. The majority (69%) of the 36 loci deviated significantly from Hardy–Weinberg equilibrium. The artificial selection pressure and development of the self-bred line may explain this phenomenon. Of the 36 markers, 10(28%), 7(19%), 7(19%), and 11(31%) were significantly associated with body weight, body length, body height, and body thickness, respectively. Eleven dominant genotypes were identified via the multiple comparisons. The higher screening rate of SSR markers tightly linked with growth traits demonstrated that the scale genes maybe associated with growth traits.

-----参考文献:---------------------------------------------------------------------------------------

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若需在您的论文中引用此文,请按以下格式著录参考文献:
中文著录格式: 肖同乾1, ,2, ,鲁翠云2, ,李超2, ,曹顶臣2, ,程磊2, ,孙效文2

.源于鳞被相关基因的微卫星标记与鲤 4 种生长性状的相关性分析.中国水产科学.2014;21(5):883-892.
英文著录格式: Xiao ,Tongqian1,2, ,Lu ,Cuiyun2, ,Li ,Chao ,2, ,Cao ,Dingchen2, ,Cheng ,Lei ,2, ,Sun ,Xiaowen2

.Correlation analysis of microsatellite markers derived from scale genes (ant, eda, edar, fgfr) with four growth traits in common carp (Cyprinus carpio) .No Title Settings.2014;21(5):883-892.

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