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鲤多拷贝基因Apo-14kDaApoE的系统学分析和其在胚胎发育中的功能探索

[中文引用][英文引用]

Phylogenetic analysis and expression during embryonic development of multiple copies of Apo-14 kDa and ApoE genes in common carp, Cyprinus carpio

作者: style="MARGIN: 0cm 0cm 5pt"> color=#000000> style="FONT-FAMILY: 华文仿宋  FONT-SIZE: 12pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'">崔润滋 face="Times New Roman"> style="FONT-SIZE: 12pt" lang=EN-US>1 2 style="FONT-SIZE: 12pt" lang=EN-US>   style="FONT-FAMILY: 华文仿宋  FONT-SIZE: 12pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'">万玉美 face="Times New Roman"> style="FONT-SIZE: 12pt" lang=EN-US>1 style="FONT-SIZE: 12pt" lang=EN-US>   style="FONT-FAMILY: 华文仿宋  FONT-SIZE: 12pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'">孙金生 face="Times New Roman"> style="FONT-SIZE: 12pt" lang=EN-US>2 style="FONT-SIZE: 12pt" lang=EN-US>   style="FONT-FAMILY: 华文仿宋  FONT-SIZE: 12pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'">汪金兔 face="Times New Roman"> style="FONT-SIZE: 12pt" lang=EN-US>1 3 style="FONT-SIZE: 12pt" lang=EN-US>   style="FONT-FAMILY: 华文仿宋  FONT-SIZE: 12pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'">徐鹏 face="Times New Roman"> style="FONT-SIZE: 12pt" lang=EN-US>1 style="FONT-SIZE: 12pt" lang=EN-US>   style="FONT-FAMILY: 华文仿宋  FONT-SIZE: 12pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'">孙效文 style="FONT-SIZE: 12pt" lang=EN-US> face="Times New Roman">1 style="LETTER-SPACING: 0pt" lang=EN-US> prefix = o ns = "urn:schemas-microsoft-com:office:office" /> 
单位(英文):1. The Centre for Applied Aquatic Genomics  Chinese Academy of Fishery Sciences  Beijing 100141  China  
分类号:S917
出版年·卷·期(页码):2012·19·第4期(741-755)
DOI: 10.3724/SP.J.1118.2012.00741
-----摘要:-------------------------------------------------------------------------------------------

本研究首次从鲤(Cyprinus carpio)中得到2种形式的14 kD载脂蛋白(Apo-14kD)和4种形式的载脂蛋白E(ApoE), 分别为Apo-14kDa-a和Apo-14kDa-b(同源性为79%)、ApoE-a1和ApoE-a2(同源性为91%)以及ApoE-b1和ApoE-b2(同源性为90%)。系统学分析表明, Apo-14kDa-a与多数硬骨鱼的Apo-14kDa同源性高于与Apo-14kDa-b的同源性, 而Apo-14kDa-b与草鱼(Ctenopharyngodon idellus)的Apo-14kDa同源性较高, 提示二者在鲤科鱼类中的分化时间较早; ApoE-a和ApoE-b的分化时间也应该处于鲤科鱼类分化的早期阶段, 随后进一步分化出4个同源基因。半定量RT-PCR结果显示Apo-14kDa-a基因在肝、头肾中大量表达, 肠中微弱表达, 其他组织无表达, Apo-14kDa-b基因只在肝表达; ApoE-a1基因和ApoE-a2基因只在肠表达; 而ApoE-b1基因和ApoE-b2基因均在脑、皮肤、鳃、肝、精巢、肠、心脏、肌肉、体肾、头肾中表达, 但在脾和卵巢发生表达分化。进一步的qRT-PCR检测发现6个基因在不同胚胎发育时期的表达模式也有较大不同, 且在不同时期的表达均存在阶段差异性。另外, qRT-PCR分析证实Apo-14kDa基因和ApoE基因均有可能在鲤胚胎发育过程中发挥着重要且显著不同的作用。

-----英文摘要:---------------------------------------------------------------------------------------

Apolipoproteins, the protein moieties of lipoproteins, participate in physical activities in different forms. A 14 kDa apolipoprotein (Apo-14 kDa) and apolipoprotein E (ApoE) are thought to be associated with embryonic development in animals. We analyzed the gene structure and phylogenetic evolution of these two types of apolipoprotein in the common carp (Cyprinus carpio). In addition, we documented the pattern of expression using RT-PCR to provide insight into their function during the embryonic development of carp. We obtained two types of Apo-14 kDa and four types of ApoE, including Apo-14kDa-a and Apo-14kDa-b (identity=79%), ApoE-a1 and ApoE-a2 (identity=91%), and ApoE-b1 and ApoE-b2 (identity=90%). Phylogenetic analysis revealed that Apo-14 kDa-a has a close relationship with that of most other teleosts and that Apo-14 kDa-b has the closest relationship with that of grass carp. These relationships likely reflect the early differentiation of the family cyprinidae, although ApoE is characterized by four homologous genes. The expression of carp Apo-14 kDa-a was highest in the liver and head-kidney, was very low in the intestine, and undetectable in the remaining tissues. Conversely, we were only able to detect expression of Apo-14 kDa-b in the liver. We observed tissue specific differences in the expression of Apo-14 kDa-a and Apo-14 kDa-b. ApoE-a1 and ApoE-a2 were only detected in the intestine whereas ApoE-b1 and ApoE-b2 were present in the brain, skin, gill, liver, spermary, intestine, heart, muscle, body-kidney, and head-kidney. ApoE-b1 was also present in the spleen and ApoE-b2 in the ovary. Our observations suggest there is considerable variation in the expression and function of ApoE. Furthermore, qRT-PCR analysis suggests that the expression of these six genes varies throughout embryonic development, both within and among genes. This is consistent with the molecular phylogeny of these genes. qRT-PCR analysis suggests that Apo-14 kDa and ApoE play an important role throughout different stages of embryonic development in common carp.

-----参考文献:---------------------------------------------------------------------------------------

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若需在您的论文中引用此文,请按以下格式著录参考文献:
中文著录格式: 崔润滋1,2,,万玉美1,,孙金生2,,汪金兔1,3,,徐鹏1,,孙效文1.

鲤多拷贝基因Apo-14kDaApoE的系统学分析和其在胚胎发育中的功能探索

.中国水产科学.2012;19(4):741-755.
英文著录格式: CUI,Runzi1,2,,WAN,Yumei1,,SUN,Jinsheng2,,WANG,Jintu1,3,,XU,Peng1,,SUN,Xiaowen1

.Phylogenetic analysis and expression during embryonic development of multiple copies of Apo-14 kDa and ApoE genes in common carp, Cyprinus carpio .No Title Settings.2012;19(4):741-755.

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