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南海短尾大眼鲷线粒体Cytb基因序列及种群判别分析 [中文引用][英文引用]

Sequence analysis of the mitochondrial cytochrome b gene and identification of the Priacanthus macracanthus population in the South China Sea

作者:熊丹1 2  李敏1  李永振3  李玉芳1 2  张魁1  陈作志1 
作者(英文):XIONG Dan1 2  LI Min1  LI Yongzhen3  LI Yufang1 2  ZHANG Kui1  CHEN Zuozhi1 
分类号:S931
出版年·卷·期(页码):2016·23·第1期(188-197)
DOI: 10.3724/SP.J.1118.2016.15114
-----摘要:-------------------------------------------------------------------------------------------

对采集于中国南海北部近岸和南沙西南部陆架海域7个采样点共246尾短尾大眼鲷(Priacanthus macracanthus)样品进行了线粒体细胞色素b基因(cytochrome b, cyt b)序列的扩增与分析,获得了长度为684 bp的同源序列。碱基A、T、C、G含量分别为22.7%、28.4%、33.4%、15.5%,共检测到多态位点92个,定义了90个单倍型。其遗传多样性表现出高单倍型多样性(0.8130~0.9012)和低核苷酸多样性(0.0040~0.0053)的特点。两两群体间的Fst分析显示,大部分群体间的Fst值均小于0.05,且差异不显著(P > 0.05),样本总体分化指数仅为0.012,群体间分化程度很低。分子方差分析(AMOVA)表明,群体间的遗传变异仅占总遗传变异的1.25%, 98.75%的遗传变异源于群体内,群体之间有较高的遗传同质性。基于邻接法构建的系统发育树和基于中间连接法构建的单倍型网络图均显示,南海短尾大眼鲷不存在与地理群体对应的支系,遗传分化不显著。中性检测和不对称分布分析发现,南海短尾大眼鲷群体在更新世晚期发生过种群扩张。结果表明南海短尾大眼鲷群体间的遗传交流较频繁,可以认为是一个大的种群。

-----英文摘要:---------------------------------------------------------------------------------------

Understanding the population structure of fish species could provide the basic information for utilization and management of fishery. The Red bigeye Priacanthus macracanthus was one of the economically important fish species that was widely distributed in the South China Sea. However, little was known about the populations. In the present study, the population genetic structure and diversity of P. macracanthus were examined in the South China Sea based on a 684 bp segment of the mitochondrial cytochrome b gene. A total of 264 individual samples were collected from seven localities in the northern South China Sea and the Southwestern Nansha Islands. The bases A, T, C, and G had frequencies of 22.7%, 28.4%, 33.4%, and 28.4%, and A + T content was similar to that of C + G (51.1% and 48.9%, respectively). Ninety-two polymorphic loci were detected and 90 haplotypes were defined. Genetic diversity was high for haploid type (0.8130-0.9012) but nucleotide diversity was low (0.0040-0.0053). A haploid type network diagram found advantages for the Hap1, Hap2, Hap4, and Hap6 haploid types, but no significant geographical features were detected. A pairwise Fst analysis showed that most of the Fst value were low and non-significant (P > 0.05). The overall Fst for the total sample was only 0.012, which showed little differentiation among populaitons. Analysis of molecular variance (AMOVA) indicated that most of the differentiation occurred within populations (98.75%) and only 1.25% occurred among populations. Phylogenetic tree constructed using neighbor-joining method showed that the seven groups of P. macracanthus were a panmictic population, with no obvious type branches. Haplotype network using median-joining network approach also revealed no significant genealogical clades corresponding to sampling localities. Both neutrality tests and mismatch distribution analyses indicated a recent population expansion in P. macracanthus during the last glacial maximum. These results indicate high gene flow among populations of P. macracanthus and they may belong to a single population in the South China Sea.

-----参考文献:---------------------------------------------------------------------------------------

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若需在您的论文中引用此文,请按以下格式著录参考文献:
中文著录格式: 熊丹1,2,,李敏1,,李永振3,,李玉芳1,2,,张魁1,,陈作志1.南海短尾大眼鲷线粒体Cytb基因序列及种群判别分析.中国水产科学.2016;23(1):188-197.
英文著录格式: XIONG,Dan1,2,,LI,Min1,,LI,Yongzhen3,,LI,Yufang1,2,,ZHANG,Kui1,,CHEN,Zuozhi1.Sequence analysis of the mitochondrial cytochrome b gene and identification of the Priacanthus macracanthus population in the South China Sea.No Title Settings.2016;23(1):188-197.

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