首页期刊介绍全国理事会投稿要求新闻动态期刊目录广告征订留言板联系我们旧系统入口
  您当前的位置:首页 >> 正文

PHB剂量和饲喂时间对中华绒螯蟹肝胰腺酶活力和肠道菌群多样性的影响 [中文引用][英文引用]

Effect of dietary PHB dose and feeding duration on enzyme activities and gut microbial diversity in juvenile Chinese mitten crab (Eriocheir sinensis)

作者(英文):DENG Yuangao1  HUANG Qiongye1  MA Guannan1  WANG Xiaomei2  SUI Liying1 
分类号:S963
出版年·卷·期(页码):2016·23·第1期(138-145)
DOI: 10.3724/SP.J.1118.2016.15077
-----摘要:-------------------------------------------------------------------------------------------

在配合饲料中添加不同剂量聚β-羟基丁酸酯(Poly-β-hydroxybutyrate, PHB)(0%、3%、5%和10%PHB)并饲喂不同时长(1 d、6 d、15 d和21 d),研究PHB对中华绒螯蟹(Eriocheir sinensis)幼蟹肝胰腺生化组成、酶活力和肠道菌群多样性的影响。结果表明, PHB对中华绒螯蟹幼蟹肝胰腺粗蛋白、粗脂肪、可溶性蛋白含量、总超氧化物歧化酶(T-SOD)和各种消化酶活力,以及肠道菌群多样性均产生一定影响,并且这种影响与饲料中PHB水平和饲喂时间有关。与对照组相比,投喂第1天, 10%PHB使T-SOD、淀粉酶和脂肪酶活力显著提高(P < 0.05), 5%和10% PHB使肠道菌群丰富度指数(Rr)显著提高(P < 0.05)。投喂第6天, 10%PHB使淀粉酶活力显著降低(P < 0.05), 3%和5%PHB使脂肪酶活力显著降低(P < 0.05),所有剂量PHB的添加均使Rr显著升高(P < 0.05)。投喂第15天, 10%PHB使T-SOD降低(P < 0.05), 5%和10%PHB使脂肪酶活力显著提高(P < 0.05),所有剂量PHB的添加使淀粉酶活力显著降低(P < 0.05),使Rr显著升高(P < 0.05)。投喂第21天,所有剂量PHB的添加使T-SOD、淀粉酶和胰蛋白酶活力显著降低(P < 0.05),而且降低幅度随PHB添加量增加而加大; 10%PHB添加显著降低Rr(P < 0.05), 3%和5%PHB添加对Rr无显著影响(P > 0.05)。因此,饲料中PHB剂量较低时,可饲喂较长时间; PHB剂量较高时,应适当缩短饲喂时间。

-----英文摘要:---------------------------------------------------------------------------------------

Poly-β-hydroxybutyrate (PHB) is a microbial storage compound that occurs in the presence of excess carbon source. PHB is degraded into water-soluble β-hydroxybutyric acid in the gut of aquatic animals and slightly lowers gut pH. The acidic gut environment benefits growth of probiotic bacteria (particularly Gram-positive bacteria), which increases enzyme secretion and improves nutrient absorption and immunity of the animal. Dietary PHB benefits the growth and survival of marine fish and crustaceans, such as European seabass, Dicentrarchus labrax, Siberian sturgeon, Acipenser baerii, and giant freshwater prawn, Macrobrachium rosenbergii. Our previous studies indicated that PHB-enriched rotifers and Artemia nauplii significantly improve molting, survival, and tolerance to vibriosis challenge in Chinese mitten crab, Eriocheir sinesis zoeal larvae, and that PHB supplementation in formulated feed improves molting of juvenile E. sinensis at the optimal dietary level of 1% PHB. However, as PHB is an organic acid-releasing compound, little is known about its possible acidosis effect during long-term feeding or the interaction between feeding duration and dietary PHB level. In this study, the dosing and feeding period effects of dietary PHB on hepatopancreatic biochemical composition and enzyme activities, as well as intestinal microbial diversity in juvenile E. sinensis, were studied by feeding formulated diets containing 0%, 3%, 5%, and 10% PHB for 1, 6, 15, and 21 days, respectively. The results showed that dietary PHB supplementation affected crude protein content, crude lipid content, soluble protein content, and various hepatopancreatic digestive enzymes, as well as microbial diversity in the gastro-intestine, which were PHB-dose and feeding-period dependent. After 1 day of feeding, the 10% PHB supplemented group had significantly higher total superoxide dismutase (T-SOD) and hepatopancreatic amylase and lipase activities compared with those in the control group fed a diet containing no PHB (P < 0.05). In addition, 5% and 10% PHB resulted in significantly higher range-weighted richness (Rr) of the gastro-intestine microbial community (P < 0.05). After 6 days of feeding, the 10% PHB treatment resulted in significantly lower amylase activity and the 3% and 5% PHB treatments resulted in significantly lower lipase activity (P < 0.05). All PHB supplemented groups had significantly higher Rr values(P < 0.05). On day 15, the 10% PHB treatment resulted in significantly lower T-SOD, and the 5% and 10% PHB treatments resulted in significantly higher lipase activity (P < 0.05). All PHB supplemented groups had significantly lower amylase activity but higher Rr values (P < 0.05). On day 20, all PHB supplemented groups had significantly reduced T-SOD, amylase, and pepsin activities (P < 0.05); the higher the PHB dose, the greater the reduction. The 10% PHB group had a significantly reduced Rr value(P < 0.05), whereas 3% and 5% PHB had no effect on the Rr value(P > 0.05). These results suggest that a longer feeding period could be applied with a lower PHB dose and vice versa to support use of dietary PHB in cultured E. sinensis.

-----参考文献:---------------------------------------------------------------------------------------

欢迎阅读《中国水产科学》!您是该文第 1048 位读者!

若需在您的论文中引用此文,请按以下格式著录参考文献:
中文著录格式: 邓元告1,,黄琼叶1,,马灌楠1,,王晓梅2,,隋丽英1.PHB剂量和饲喂时间对中华绒螯蟹肝胰腺酶活力和肠道菌群多样性的影响.中国水产科学.2016;23(1):138-145.
英文著录格式: DENG,Yuangao1,,HUANG,Qiongye1,,MA,Guannan1,,WANG,Xiaomei2,,SUI,Liying1.Effect of dietary PHB dose and feeding duration on enzyme activities and gut microbial diversity in juvenile Chinese mitten crab (Eriocheir sinensis).No Title Settings.2016;23(1):138-145.

与该文相关的文章(仅限于本刊内

已投本刊未发表相似文章

《中国人口科学》编辑部 © 2011
地址:北京市三里河路9号建设部北配楼449房间
联系电话: (010)85195419 电子邮件: zazhi@cass.org.cn
电子邮件:rkkx@sina.com