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碳酸盐碱度胁迫下瓦式雅罗鱼血浆游离氨基酸水平的变化 [中文引用][英文引用]

Changes in plasma free amino acid levels in Leuciscus waleckii exposed to different environmental alkalinity levels

作者(英文):CHANG Yumei1  HE Qiang1 2  SUN Yanchun1  LIANG Liqun1  SUN Xiaowen1 
分类号:S965
出版年·卷·期(页码):2016·23·第1期(117-124)
DOI: 10.3724/SP.J.1118.2016.15080
-----摘要:-------------------------------------------------------------------------------------------

为了从不同层面解析达里湖瓦氏雅罗鱼(Leuciscus waleckii)耐高碱的生理机制,本研究对达里湖瓦氏雅罗鱼在2个时间段(22 d和60 d)和3个不同浓度碳酸盐碱度(18 mmol/L、30 mmol/L和50 mmol/L)胁迫下的15种血浆游离氨基酸(PFAAs)含量的变化趋势进行了分析。结果显示,血浆PFAAs总量随着碱度的上升而显著增加,而且随着胁迫时间的延长,由必需氨基酸增量为主转向非必需氨基酸增量为主;作为能量代谢的主要底物,非必需氨基酸中的脯氨酸(Pro)和缬氨酸(Val)含量随着碱度的增加而显著增加(P < 0.05),尤其是Pro在碱度胁迫组中的含量增幅为对照组的23倍。本研究结果表明,达里湖瓦氏雅罗鱼在高碱环境胁迫下,提高血浆中非必需氨基酸的含量作为能量来源,可能是其适应高碱环境的主要策略之一。同时,初步探讨了发生显著变化的游离氨基酸可能参与的生理过程,为从氨基酸代谢组学角度解析瓦氏雅罗鱼耐高碱的生理和分子机制提供了科学依据。

-----英文摘要:---------------------------------------------------------------------------------------

<i>Leuciscus waleckii</i> is a cyprinid in the subfamily Leuciscinae and is distributed widely in rivers and lakes of Northeast, Northwest, and Northern China. A unique feature of this species is its resistance to high alkalinity, which allows it to survive and adapt to the extreme environment of Lake Dali, Inner Mongolia, with carbonate alkalinity > 53.57 mmol/L (pH 9.6). Previous studies have explored the mechanisms of the extreme alkaline tolerance using physiological, chemical, population genetics, and transcriptomics methods. We assessed the effects of acclimating <i>L. waleckii</i> to different environmental alkalinity levels and exposure times on plasma free amino acids levels. <i>L. waleckii</i> juveniles were acclimated to alkalinities of 18 mmol/L, 30 mmol/L, and 50 mmol/L for 22 d and 60 d, and plasma levels of 15 free amino acids were examined and compared between the different alkalinity groups and the control. The results showed that total plasma free amino acid contents increased with alkalinity and exposure time, and that the levels of nonessential amino acids changed the most. Among the nonessential amino acids, proline and valine contents increased significantly with increasing alkalinity (<i>P</i> < 0.05); proline increased 23 times more than the control in the 50 mmol/L alkalinity group. These results suggest that amino acids play an important role as an energy source during acclimation of <i>L. waleckii</i> to high alkalinity conditions.

-----参考文献:---------------------------------------------------------------------------------------

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若需在您的论文中引用此文,请按以下格式著录参考文献:
中文著录格式: 常玉梅1,,何强1,2,,孙言春1,,梁利群1,,孙效文1.碳酸盐碱度胁迫下瓦式雅罗鱼血浆游离氨基酸水平的变化.中国水产科学.2016;23(1):117-124.
英文著录格式: CHANG,Yumei1,,HE,Qiang1,2,,SUN,Yanchun1,,LIANG,Liqun1,,SUN,Xiaowen1.Changes in plasma free amino acid levels in Leuciscus waleckii exposed to different environmental alkalinity levels.No Title Settings.2016;23(1):117-124.

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