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诺氟沙星在西伯利亚鲟体内蓄积规律及其对组织的病理损伤 [中文引用][英文引用]

Norfloxacin accumulation rule and toxicity to Siberian sturgeon (Acipenser baerii Brandt)

作者:王荻1  李绍戊1  冯娟2 3  卢彤岩1 
作者(英文):WANG Di1  LI Shaowu1  FENG Juan2 3  LU Tongyan1 
关键词(英文):norfloxacin  Siberian sturgeon  accumulation rule  toxicity 
分类号:S948
出版年·卷·期(页码):2015·22·第6期(1209-1218)
DOI: 10.3724/SP.J.1118.2015.15021
-----摘要:-------------------------------------------------------------------------------------------

为了确定诺氟沙星在鲟养殖中的科学使用方法, 对不同剂量、不同给药次数情况下诺氟沙星在西伯利亚鲟(<i>Acipenser baerii</i>)体内蓄积规律及毒性进行了研究.分别以0、30 mg/kg、50 mg/kg、100 mg/kg的剂量对西伯利亚鲟连续口灌诺氟沙星3~5 d, 每天1次, 并于停药后24 h及240 h采集实验鱼血、肝、肾及软骨组织, 对4种组织中药物蓄积量进行测定, 并对肝、肾和软骨组织进行切片观察.实验结果表明: 随着给药剂量增加、给药次数增多, 实验鱼4种组织中诺氟沙星浓度逐渐升高, 浓度由高到低依次为为: 肾、软骨、肝、血清, 且血清中药物浓度远远低于另外3种组织.相同给药剂量、不同给药次数时血清中药物浓度无显著差异; 而肝、肾和软骨中均差异显著, 停药240 h后, 显著性消失.切片结果表明, 50 mg/kg剂量连续给药5 d后, 实验鲟肝、肾组织开始结构损伤变化, 100 mg/kg剂量连续给药3~5 d后, 损伤情况持续加重, 停药240 h后, 肝组织损伤情况有逐渐恢复趋势, 而肾组织未见明显恢复趋势; 而100 mg/kg连续给药3~5 d后, 软骨组织出现软骨膜内层细胞减少; 停药240 h后有恢复趋势.研究结果表明, 诺氟沙星在鲟中使用剂量应小于50 mg/kg, 连续给药次数低于5次, 以避免对鲟肝组织造成损伤, 引起肾不可逆损伤及影响软骨细胞发育和再生, 对软骨组织造成潜在影响.

-----英文摘要:---------------------------------------------------------------------------------------

Norfloxacin is an efficacious broad-spectrum antibiotic that has been used widely to prevent and control fish bacterial diseases. However, no comprehensive study has examined the toxicity of norfloxadin to aquatic animals. Siberian sturgeon is the principle sturgeon species cultured in China and has high scientific research and production value. Quinolones occasionally cause an abnormal spinal column in Siberian sturgeon when used to control bacterial diseases. Thus, we investigated the accumulation rule and toxicity of different norfloxadin dosages and exposure times in Siberian sturgeon. Norfloxacin was given orally to experimental fish at dosages of 0, 30 mg/kg, 50 mg/kg, and 100 mg/kg for 3-5 d. Blood, liver, kidney, and cartilage were collected for 24-240 h. The quantities of drug retained in the four tissues were determined, and histological sections of the liver, kidney, and cartilage were observed. The results showed that the concentrations of norfloxacin in the four Siberian sturgeon tissues increased gradually with increasing norfloxadin dosage and time in a trend of kidney > cartilage > liver > serum. Significant differences in liver, kidney, and cartilage drug concentrations were detected when norfloxadin was given to fish at the same dosage but for different durations. These differences disappeared after withdrawal for 240 h. The histology indicated that the liver and kidney were damaged when norfloxadin was given to fish at 50 mg/kg for 5 d, and the damage appeared to be more serious at 100 mg/kg for 3-5 d. Damage to the liver, but not the kidney, recovered gradually during the 240 h withdrawal. The number of chondrocytes near the perichondrium decreased in cartilage when norfloxadin was given at 100 mg/kg for 3-5 d but recovered gradually during the 240 h withdrawal. These results suggest that the norfloxadin dosage should be no more than 50 mg/kg and that exposure time should be <5 d to prevent liver, kidney, and cartilage damage. Based on these results, norfloxadin exhibited potential damage to the liver, kidney, and cartilage in Siberian sturgeon, suggesting that the dosing regimen must be strictly followed in sturgeon aquaculture. Further study is needed to describe norfloxadin metabolism, residue, and damage repair. Suitable Chinese herbal medicines could be used to promote cartilage differentiation and repair.

-----参考文献:---------------------------------------------------------------------------------------

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若需在您的论文中引用此文,请按以下格式著录参考文献:
中文著录格式: 王荻1,,李绍戊1,,冯娟2,3,,卢彤岩1.诺氟沙星在西伯利亚鲟体内蓄积规律及其对组织的病理损伤.中国水产科学.2015;22(6):1209-1218.
英文著录格式: WANG,Di1,,LI,Shaowu1,,FENG,Juan2,3,,LU,Tongyan1.Norfloxacin accumulation rule and toxicity to Siberian sturgeon (Acipenser baerii Brandt).No Title Settings.2015;22(6):1209-1218.

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