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抗迟缓爱德华氏菌病牙鲆家系的筛选与分析 [中文引用][英文引用]

Screening for resistance to Edwardsiella tarda in different families of Japanese flounder, Paralichthys olivaceus

作者(英文):SUN Hejun1 2  CHEN Songlin1 3  ZHENG Weiwei1 2  MA Jalu1  WANG Wenwen1  TIAN Yongsheng1  DENG Han1  CHEN Honglin1 2  GAO Jin1 
关键词(英文):Paralichthys olivaceus  Edwardsiella tarda  resistance  family screening 
分类号:S94
出版年·卷·期(页码):2015·22·第6期(1115-1122)
DOI: 10.3724/SP.J.1118.2015.15048
-----摘要:-------------------------------------------------------------------------------------------

牙鲆(<i>Paralichthys olivaceus</i>)养殖病害日益严重, 迟缓爱德华氏菌(<i>Edwardsiella tarda</i>)是牙鲆养殖中的主要致病菌, 带来了极大的经济损失, 为了培育出对该病抵抗能力强的牙鲆新品种, 2014年4-6月间, 利用我们长期以来建立和筛选出来的牙鲆抗病群体和家系的亲鱼, 通过杂交, 自交, 雌核发育建立47个家系, 9-10月间, 对其中39个家系进行感染实验, 先用少量鱼进行预实验摸索出半致死浓度, 然后再进行正式感染实验, 感染数量为每个家系80尾.各家系的存活率范围为1.19%~51.19%, 最终平均存活率为20.29%, 认为存活率高于30%的7个家系为抗病力强家系, 存活率在20.29%~30%的9个家系为抗病力一般家系, 平均存活率以下的23个家系为抗病力弱家系.1406<sup>#</sup>家系抗病力最强, 它为1005<sup>#</sup>家系的自交后代, 而其他抗病力强家系也大多是1005<sup>#</sup>、09104<sup>#</sup>、0915<sup>#</sup>的后代.而09104<sup>#</sup>为0768<sup>#</sup>的后代, 并且0768<sup>#</sup>抗鳗弧菌病能力强.1005<sup>#</sup>为韩国群体自交后代, 0915<sup>#</sup>为韩国群体和日本群体杂交后代.这些抗病力强的牙鲆可以作为新品系进行推广养殖, 可望减少牙鲆腹水病发生.

-----英文摘要:---------------------------------------------------------------------------------------

<i>Edwardsiella tarda</i> is a significant pathogenic bacterium that causes considerable economic losses in flounder aquaculture. We created 47 flounder families using parental fish resistant to <i>E. tarda</i> and screened them long term through cross-fertilization, self-crossing, and gynogenesis experiments during April-June 2014. We infected 39 flounder families with <i>E. tarda</i> from June-October. The half lethal bacterial concentration was determined to be 80 in each family using a small number of flounder in a preliminary experiment. Survival rate was 1.19%-51.19% (mean, 20.29%). Seven families with survival rates >30% were most able to resist <i>E. tarda</i>; the nine families with survival rates of 20.29%-30% had normal resistance to <i>E. tarda</i>; and the 23 families with survival rates <20.29% were least resistant to <i>E. tarda</i>. Family 1406 had the best resistance, which was self-crossed with 1005. The families with the best resistance were all descendants of families 1005, 09104, and 0915. Family 09104 was a descendant of family 0768, which was resistant to <i>Vibrio anguillarum</i>, and was self-crossed with family 1005 and a Korean group. Family 0915 was cross-fertilized with Korean and Japanese groups. These disease-resistant flounder could be popularized as new strains to reduce the occurrence of virulent ascitesosis disease.

-----参考文献:---------------------------------------------------------------------------------------

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中文著录格式: 孙何军1,2,,陈松林1,3,,郑卫卫1,2,,马佳璐1,,王文文1,,田永胜1,,邓寒1,,陈红林1,2,,高进1.抗迟缓爱德华氏菌病牙鲆家系的筛选与分析.中国水产科学.2015;22(6):1115-1122.
英文著录格式: SUN,Hejun1,2,,CHEN,Songlin1,3,,ZHENG,Weiwei1,2,,MA,Jalu1,,WANG,Wenwen1,,TIAN,Yongsheng1,,DENG,Han1,,CHEN,Honglin1,2,,GAO,Jin1.Screening for resistance to Edwardsiella tarda in different families of Japanese flounder, Paralichthys olivaceus.No Title Settings.2015;22(6):1115-1122.

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