首页期刊介绍全国理事会投稿要求新闻动态期刊目录广告征订留言板联系我们旧系统入口
  您当前的位置:首页 >> 正文

刺参池塘低温有机污染物降解细菌的分离筛选及其降解特性 [中文引用][英文引用]

Isolation, selection and characterization of low-temperature organic- pollutant-degrading bacteria in sea cucumber ponds

作者: color=#000000> style="LINE-HEIGHT: 110%  FONT-FAMILY: 方正书宋简体  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'  mso-bidi-font-family: 'Times New Roman'  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA"> face=宋体>闫法军 style="LINE-HEIGHT: 110%  FONT-FAMILY: 'Times New Roman'  'serif'  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA  mso-fareast-font-family: 方正书宋简体" lang=EN-US>   style="LINE-HEIGHT: 110%  FONT-FAMILY: 方正书宋简体  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'  mso-bidi-font-family: 'Times New Roman'  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA"> face=宋体>田相利 style="LINE-HEIGHT: 110%  FONT-FAMILY: 'Times New Roman'  'serif'  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA  mso-fareast-font-family: 方正书宋简体" lang=EN-US>   style="LINE-HEIGHT: 110%  FONT-FAMILY: 方正书宋简体  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'  mso-bidi-font-family: 'Times New Roman'  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA"> face=宋体>董双林 style="LINE-HEIGHT: 110%  FONT-FAMILY: 'Times New Roman'  'serif'  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA  mso-fareast-font-family: 方正书宋简体" lang=EN-US>   style="LINE-HEIGHT: 110%  FONT-FAMILY: 方正书宋简体  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'  mso-bidi-font-family: 'Times New Roman'  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA"> face=宋体>武心华 style="LINE-HEIGHT: 110%  FONT-FAMILY: 'Times New Roman'  'serif'  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA  mso-fareast-font-family: 方正书宋简体" lang=EN-US>   style="LINE-HEIGHT: 110%  FONT-FAMILY: 方正书宋简体  LETTER-SPACING: 0.2pt  FONT-SIZE: 10.5pt  mso-ascii-font-family: 'Times New Roman'  mso-hansi-font-family: 'Times New Roman'  mso-bidi-font-family: 'Times New Roman'  mso-font-kerning: 1.0pt  mso-ansi-language: EN-US  mso-fareast-language: ZH-CN  mso-bidi-language: AR-SA"> face=宋体>柳柄俊 
分类号:S917.1
出版年·卷·期(页码):2012·19·第4期(626-634)
DOI: 10.3724/SP.J.1118.2012.00626
-----摘要:-------------------------------------------------------------------------------------------

利用刺参(Apostichopus japonicus)饵料培养基, 在低温(16±1)℃下, 从刺参养殖池塘环境中富集驯化分离有机污染物低温降解细菌; 根据菌株生长情况、好氧性和生物安全性进行了初步筛选, 然后在菌体接种密度1×106 CFU/mL、(16±1)℃、pH8.0、160 r/min条件下, 通过检测菌株对饵料中有机物(COD)和氨态氮(NH4+-N)成分的去除率来最终筛选获得优良低温有机污染物降解细菌, 并对其降解特性进行研究。菌株筛选结果表明, 从分离的17株低温细菌中共获得3株菌株DR7、DR8和DR11, 它们能同时高效降低和去除饵料浸出物中COD和NH4+-N, 5dCOD去除率分别为37.7%、50.0%和21.0%, NH4+-N去除率分别为95.0%、98.0%和99.8%。根据菌株的形态特征、生理生化特性以及16S rRNA序列分析, 初步鉴定DR7和DR11属于假单胞菌属细菌(Pseudomonas sp.), DR8属于副球菌属细菌(Paracoccus sp.)。菌株降解特性研究结果表明, 菌株培养时间对饵料降解有显著影响(P<0.05), 在0~6d内3菌株对饵料中有机物和氨态氮的去除过程明显, 其中DR8降解力最强; 各组合菌株的饵料降解效果差异显著(P<0.05), 但并不完全优于对照单菌株, 其中最佳组合为DR8-DR11。研究认为, 筛选的3株低温有机污染物降解细菌为良好的菌种资源, 在刺参养殖池塘污染环境调控及刺参微生态制剂的研制方面具有较好的潜在应用前景

-----英文摘要:---------------------------------------------------------------------------------------

Low-temperature organic-pollutant-degrading bacteria were isolated from sea cucumber (Apostichopus japonicus) outdoor rearing ponds after enriching the liquid sea cucumber feed-medium and acclimating at (16±1)℃. All of the bacterial strains were preliminarily selected by evaluating their aerobic growth on sea cucumber feed-medium and verifying their specificity for the sea cucumber. The bacteria were finally selected by evaluating their ability to degrade organic pollutants (COD) and ammonia nitrogen (NH4+-N) in the feed-medium, with an inoculation of 1.0×106 cfu/mL. Culture conditions were (16±1)℃, pH 8.0 and a shaking rate of 160 r/min. After screening of 17 bacterial isolates, three bacterial strains (DR7, DR8 and DR11) were selected. These strains were able to degrade the feed over 5 d with COD removal rates of 37.7%, 50.0% and 21.0%, and NH4+-N removal rates of 95.0%, 98.0% and 99.8%, respectively. From morphological and physio-biochemical analyses, and 16S rRNA sequence similarity analysis, strains DR7 and DR11 were identified as Pseudomonas sp., and strain DR8 as Paracoccus sp. The time course of degradation of the feed was observed by examination of the concentrations of the organic pollutants and ammonia-nitrogen in the liquid sea cucumber-feed-medium between 0 d and 6 d. Bacterial cultivation time had a significant effect on the feed-degrading activities of the three strains (P<0.05). Based on the dynamics of feed degradation, strain DR8 was considered to be the most effective of the three strains. Significant differences (P<0.05) were also observed in the combined feed-degrading capacities of mixtures of the strains. The optimal combination was considered to be DR8 + DR11. However, the overall feed-degrading abilities of the combined strains were not much better than that of the single strain. We conclude that the 3 low-temperature organic-pollutant-degrading bacteria screened in this study are useful bacterial resources, which could show good potential for pollution control in sea cucumber culture ponds and the development of probiotics for sea cucumber culture.

-----参考文献:---------------------------------------------------------------------------------------

欢迎阅读《中国水产科学》!您是该文第 2406 位读者!

若需在您的论文中引用此文,请按以下格式著录参考文献:
中文著录格式: 闫法军,,田相利,,董双林,,武心华,,柳柄俊.刺参池塘低温有机污染物降解细菌的分离筛选及其降解特性.中国水产科学.2012;19(4):626-634.
英文著录格式: YAN,Fajun,,TIAN,Xiangli,,DONG,Shuanglin,,WU,Xinhua,,LIU,Bingjun.Isolation, selection and characterization of low-temperature organic- pollutant-degrading bacteria in sea cucumber ponds.No Title Settings.2012;19(4):626-634.

与该文相关的文章(仅限于本刊内

已投本刊未发表相似文章

《中国人口科学》编辑部 © 2011
地址:北京市三里河路9号建设部北配楼449房间
联系电话: (010)85195419 电子邮件: zazhi@cass.org.cn
电子邮件:rkkx@sina.com